Wissenschaftlicher Mitarbeiter und Doktorand
Gerassimos Kolaitis, M.Sc.
Wissenschaftlicher Mitarbeiter und Doktorand
Tel. Büro: +49 (0)89 289 – 13171
Tel.Labor: +49 (0)89 289 – 54209
Fax: +49 (0) 9421 187-310
E-Mail: gerassimos.kolaitis@tum.de
Chair of Chemistry of Biogenic Resources
Catalysis Research Center
Ernst-Otto-Fischer-Straße 1
85748 Garching
Raum: 2018
Vita
Curriculum
Since August 2018: PhD student at the Chair of Chemistry of Biogenic Resources
2018: Master’s degree at the Technical University of Munich (Industrial Biotechnology)
Thesis: Cloning, Production and Functional Characterization of Expansins for Lignocellulose Degradation
2015: Bachelor degree at the Technical University of Munich (Molecular Biotechnology)
Thesis: Immobilization of eGFP on the Surface of Polymer Vesicles
Research
Location: Garching lab, Method development for synthetic biology
Establishing a microfluidic platform for high throughput enzyme screening
In my PhD, I am specializing in the development of droplet-based high-throughput screening systems and assays. In droplet microfluidics, picoliter-sized droplets can be generated and analyzed at kHz speeds. These droplets serve as compartments similar to microplate wells. Microfluidics offers significantly higher screening throughputs compared to conventional methods while also decreasing overall reactant consumption to a few microliters. However, a droplet-based system has unique assay requirements. Therefore, this research focuses on developing novel assays tailored for droplet-based systems, specifically targeting the screening of enzyme variants and the identification of new enzymes within metagenomes.
Supported by BMBF and TU-Munich