Research associate

Dr. rer. nat. Broder Rühmann
Dr. rer. nat. Broder Rühmann

Research associate

Phone: +49 (0) 9421 187-332
Fax: +49 (0) 9421 187-310
Mail: broder.ruehmann@tum.de

TUM Campus Straubing
Schulgasse 16
Germany, 94315 Straubing

Schulgasse 16, Room: 1.A06

Vita

Curriculum Vitae:

  • From 2018 onwards: Group leader Exopolysaccharide; Chair of Chemistry of Biogenic Resources, Technical University of Munich

  • 2016: Wissenschaftspreis (Translation: Science Prize) of the “Hochschulstadt Straubing e.V.” in the Doctorate category

  • 2015: PhD Graduation, Dissertation Topic / Thesis Title: “Development of a Modular High Throughput Screening Platform for Microbial Exopolysaccharide Producers”

  • Rühmann B (2016): Development of a Modular High Throughput Screening Platform for Microbial Exopolysaccharide Producers. PhD thesis, In: Sieber V (Hrsg.): Technische Universität München, Nachwachsende Rohstoffe in Forschung und Praxis. Band: 22 Verlag Attenkofer, Straubing. ISBN: 978-3-942742-68-9

  • 2014-2018: Research Associate, Chair  of Chemistry of Biogenic Resources, Technical University of Munich

  • 2009-2014: PhD Student, Chair of Chemistry of Biogenic Resources, Technical University of Munich

  • 2005 -2009: Development Engineer for Biotechnology – Evonik Degussa GmbH, Germany

  • 2007: Award of the Society for the Promotion of the University of Applied Sciences Weihenstephan, Germany

  • 2005: Diploma Thesis – Evonik Degussa GmbH, Germany: Analysis of potential exopolyssaccharide (EPS) producing microorganisms and their produced EPS

  • 2003-2004: Internship – Bio-Rad Laboratories GmbH, Germany: Optimization of the HPLC-analysis of carbohydrate-deficient-transferrin (CDT) and increasing of the column lifetime

  • 2000-2005: University of Applied Sciences Weihenstephan, Graduation in Biotechnology Dipl-.Ing. (FH)

  • 1997-2000: Apprenticeship as Laboratory Assistant in Milk Science, Danisco Cultor Niebüll GmbH, Germany Tribute by the Bundesminister

Research:

  • Entwicklung einer modularen Hochdurchsatz-Screening-Plattform für Exopolysaccharide
  • Identifizierung, Fermentation und Strukturaufklärung neuer Exopolysaccharide
  • UHPLC-MS / GC-MS Methodenentwicklung

Publications:

  • Ortiz-Tena, J. G.; Rühmann, B. und Sieber, V. (2018): Colorimetric Determination of Sulfate via an Enzyme Cascade for High-Throughput Detection of Sulfatase Activity. In: Anal Chem, Vol. 90, 4, S. 2526-2533.

  • Schmid, J.; Rühmann, B.; Sieber, V.; Romero-Jimenez, L.; Sanjuan, J. und Perez-Mendoza, D. (2018): Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria. In: Methods Mol Biol, Vol. 1734, S. 263-275.

  • Rütering, Marius; Cress, Brady F.; Schilling, Martin; Rühmann, Broder; Koffas, Mattheos A. G.; Sieber, Volker und Schmid, Jochen (2017): Tailor-made exopolysaccharides—CRISPR-Cas9 mediated genome editing in Paenibacillus polymyxa. In: Synthetic Biology, Vol. 2, 1, S.

  • Koenig, Steven; Rühmann, Broder; Sieber, Volker und Schmid, Jochen (2017): Quantitative assay of β-(1,3)–β-(1,6)–glucans from fermentation broth using aniline blue. In: Carbohydrate Polymers, Vol. 174, S. 57-64.

  • Ortiz-Tena, Jose G.; Rühmann, Broder; Schieder, Doris und Sieber, Volker (2016): Revealing the diversity of algal monosaccharides: Fast carbohydrate fingerprinting of microalgae using crude biomass and showcasing sugar distribution in Chlorella vulgaris by biomass fractionation. In: Algal Research, Vol. 17, S. 227-235.

  • Rütering M, Schmid J, Rühmann B, Schilling M, Sieber V (2016) Controlled production of polysaccharides – exploiting nutrient supply for levan and heteropolysaccharide formation in Paenibacillus sp.. Carbohydrate Polymers (in press).
  • Rühmann B, Schmid J, Sieber V (2016) Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis. J Vis Exp, 110(e53249). doi:10.3791/53249
  • Rühmann, B., Schmid, J., and Sieber, V. (2015) Methods to identify the unexplored diversity of microbial exopolysaccharides, Frontiers in Microbiology 6, doi: 10.3389/fmicb.2015.00565
  • Rühmann B. , Schmid J. , Sieber V. (2015) High throughput exopolysaccharide screening platform: From strain cultivation to monosaccharide composition and carbohydrate fingerprinting in one day, doi:10.1016/j.carbpol.2014.12.021
  • Rühmann, B., Schmid, J., Sieber, V. (2014). Fast carbohydrate analysis via liquid chromatography coupled with ultra violet and electrospray ionization ion trap detection (LC-UV-ESI-MS/MS) in 96-well format, J. Chromatogr. A 1350:44-50. doi:10.1016/j.chroma.2014.05.014
  • Schmid J, Koenig S, Rühmann B, Rütering M, Sieber V (2014) Biosynthese und Genomik mikrobieller Polysaccharide. Biospektrum 20 (3):288-290. doi:10.1007/s12268-014-0443-0
  • Pick, A., Rühmann, B., Schmid, J., Sieber, V. (2012) Novel CAD-like enzymes from Escherichia coli K-12 as additional tools in chemical production, Appl.Microbiol. Biotechnol DOI: 10.1007/s00253-012-4474-5 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]
  • Jan-Karl Guterl, Daniel Garbe, Jörg Carsten, Fabian Steffler, Bettina Sommer, Steven Reiße, Anja Philipp, Martina Haack, Broder Rühmann, Ulrich Kettling, Thomas Brück & Volker Sieber (2012) Cell-free metabolic engineering – Production of chemicals via minimized reaction cascades ChemSusChem, DOI: 10.1002/cssc.20120036

Patents:

  • Volker Sieber, André Pick, Broder Rühmann (2011) Synthetic enzymatic pathway for the production of chemicals with reduced number of functional groups EP11159592.2
  • Volker Sieber, Katrin Grammann, Broder Rühmann, Thomas Haas (2010) Method for producing multicyclical ring systems carrying amino groups. WO 2010089171
  • Volker Sieber, Johannes Kaiser, Broder Rühmann et al. (2007). Manufacture of isoprenoids with microorganisms expressing plant genes for enzymes of isoprenoid biosynthesis, Evonik Degussa GmbH, Germany WO 2009030654
  • Volker Sieber, Astrid Jäger, Broder Rühmann (2007) Fermentation of biopolymers with lower acyl group content using microorganisms with increased acylase activity, Evonik Degussa GmbH, Germany DE 102007041861

Contact

Chair of Chemistry of Biogenic Resources

Schulgasse 16
D-94315 Straubing

Head

Prof. Dr. Volker Sieber

Phone: +49 (0) 9421 187-300
Phone: +49 (0) 8161 71 35 91
Mail: sieber@tum.de

Team Assistant

Elisabeth Aichner

Phone: +49 (0) 9421 187-301
Mail: elisabeth.aichner@tum.de