Curriculum Vitae:

• From 2018 onwards: Group leader Exopolysaccharide; Chair of Chemistry of Biogenic Resources, Technical University of Munich
• 2016: Wissenschaftspreis (Translation: Science Prize) of the “Hochschulstadt Straubing e.V.” in the Doctorate category
• 2015: PhD Graduation, Dissertation Topic / Thesis Title: “Development of a Modular High Throughput Screening Platform for Microbial Exopolysaccharide Producers”
• Rühmann B (2016): Development of a Modular High Throughput Screening Platform for Microbial Exopolysaccharide Producers. PhD thesis, In: Sieber V (Hrsg.): Technische Universität München, Nachwachsende Rohstoffe in Forschung und Praxis. Band: 22 Verlag Attenkofer, Straubing. ISBN: 978-3-942742-68-9
• 2014-2018: Research Associate, Chair  of Chemistry of Biogenic Resources, Technical University of Munich
• 2009-2014: PhD Student, Chair of Chemistry of Biogenic Resources, Technical University of Munich
• 2005 -2009: Development Engineer for Biotechnology – Evonik Degussa GmbH, Germany
• 2007: Award of the Society for the Promotion of the University of Applied Sciences Weihenstephan, Germany
• 2005: Diploma Thesis – Evonik Degussa GmbH, Germany: Analysis of potential exopolyssaccharide (EPS) producing microorganisms and their produced EPS
• 2003-2004: Internship – Bio-Rad Laboratories GmbH, Germany: Optimization of the HPLC-analysis of carbohydrate-deficient-transferrin (CDT) and increasing of the column lifetime
• 2000-2005: University of Applied Sciences Weihenstephan, Graduation in Biotechnology Dipl-.Ing. (FH)
• 1997-2000: Apprenticeship as Laboratory Assistant in Milk Science, Danisco Cultor Niebüll GmbH, Germany Tribute by the Bundesminister

Research:

• Entwicklung einer modularen Hochdurchsatz-Screening-Plattform für Exopolysaccharide
• Identifizierung, Fermentation und Strukturaufklärung neuer Exopolysaccharide
• UHPLC-MS / GC-MS Methodenentwicklung

Publications:

• Schulze, C., et al., Investigation of exopolysaccharide formation and its impact on anaerobic succinate production with Vibrio natriegens. Microbial Biotechnology, 2023. n/a(n/a) DOI: https://doi.org/10.1111/1751-7915.14277.

• Schilling, C., et al., CRISPR-Cas9 driven structural elucidation of the heteroexopolysaccharides from Paenibacillus polymyxa DSM 365. Carbohydrate Polymers, 2023. 312: p. 120763 DOI: https://doi.org/10.1016/j.carbpol.2023.120763.

• Sinzinger, K., et al., Towards a cyanobacterial biorefinery: Carbohydrate fingerprint, biocomposition and enzymatic hydrolysis of Nostoc biomass. Algal Research, 2022. 65: p. 102744 DOI: https://doi.org/10.1016/j.algal.2022.102744.

• Schilling, C., et al., Structural elucidation of the fucose containing polysaccharide of Paenibacillus polymyxa DSM 365. Carbohydrate Polymers, 2022. 278: p. 118951 DOI: https://doi.org/10.1016/j.carbpol.2021.118951.

• Rath, T., et al., Systematic optimization of exopolysaccharide production by Gluconacetobacter sp. and use of (crude) glycerol as carbon source. Carbohydrate Polymers, 2022. 276: p. 118769 DOI: https://doi.org/10.1016/j.carbpol.2021.118769.

• Medina-Cabrera, E.V., et al., Rheological characterization of Porphyridium sordidum and Porphyridium purpureum exopolysaccharides. Carbohydrate Polymers, 2021. 253: p. 117237 DOI: https://doi.org/10.1016/j.carbpol.2020.117237.

• Medina-Cabrera, E.V., et al., Optimization of growth and EPS production in two Porphyridum strains. Bioresource Technology Reports, 2020. 11: p. 100486 DOI: https://doi.org/10.1016/j.biteb.2020.100486.

• Medina-Cabrera, E.V., Characterization and comparison of Porphyridium sordidum and Porphyridium purpureum concerning growth characteristics and polysaccharide production. Algal research, 2020. v. 49: p. 2020 v.49 DOI: 10.1016/j.algal.2020.101931.

• Shelat, K.J., et al., Overall Nutritional and Sensory Profile of Different Species of Australian Wattle Seeds (Acacia spp.): Potential Food Sources in the Arid Semi-Arid Regions. Foods, 2019. 8(10) DOI: 10.3390/foods8100482.

• Ortiz-Tena, J. G.; Rühmann, B. und Sieber, V. (2018): Colorimetric Determination of Sulfate via an Enzyme Cascade for High-Throughput Detection of Sulfatase Activity. In: Anal Chem, Vol. 90, 4, S. 2526-2533.
• Schmid, J.; Rühmann, B.; Sieber, V.; Romero-Jimenez, L.; Sanjuan, J. und Perez-Mendoza, D. (2018): Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria. In: Methods Mol Biol, Vol. 1734, S. 263-275.
• Rütering, Marius; Cress, Brady F.; Schilling, Martin; Rühmann, Broder; Koffas, Mattheos A. G.; Sieber, Volker und Schmid, Jochen (2017): Tailor-made exopolysaccharides—CRISPR-Cas9 mediated genome editing in Paenibacillus polymyxa. In: Synthetic Biology, Vol. 2, 1, S.
• Koenig, Steven; Rühmann, Broder; Sieber, Volker und Schmid, Jochen (2017): Quantitative assay of β-(1,3)–β-(1,6)–glucans from fermentation broth using aniline blue. In: Carbohydrate Polymers, Vol. 174, S. 57-64.
• Ortiz-Tena, Jose G.; Rühmann, Broder; Schieder, Doris und Sieber, Volker (2016): Revealing the diversity of algal monosaccharides: Fast carbohydrate fingerprinting of microalgae using crude biomass and showcasing sugar distribution in Chlorella vulgaris by biomass fractionation. In: Algal Research, Vol. 17, S. 227-235.
• Rütering M, Schmid J, Rühmann B, Schilling M, Sieber V (2016) Controlled production of polysaccharides – exploiting nutrient supply for levan and heteropolysaccharide formation in Paenibacillus sp.. Carbohydrate Polymers (in press).
• Rühmann B, Schmid J, Sieber V (2016) Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis. J Vis Exp, 110(e53249). doi:10.3791/53249
• Rühmann, B., Schmid, J., and Sieber, V. (2015) Methods to identify the unexplored diversity of microbial exopolysaccharides, Frontiers in Microbiology 6, doi: 10.3389/fmicb.2015.00565
• Rühmann B. , Schmid J. , Sieber V. (2015) High throughput exopolysaccharide screening platform: From strain cultivation to monosaccharide composition and carbohydrate fingerprinting in one day, doi:10.1016/j.carbpol.2014.12.021
• Rühmann, B., Schmid, J., Sieber, V. (2014). Fast carbohydrate analysis via liquid chromatography coupled with ultra violet and electrospray ionization ion trap detection (LC-UV-ESI-MS/MS) in 96-well format, J. Chromatogr. A 1350:44-50. doi:10.1016/j.chroma.2014.05.014
• Schmid J, Koenig S, Rühmann B, Rütering M, Sieber V (2014) Biosynthese und Genomik mikrobieller Polysaccharide. Biospektrum 20 (3):288-290. doi:10.1007/s12268-014-0443-0
• Pick, A., Rühmann, B., Schmid, J., Sieber, V. (2012) Novel CAD-like enzymes from Escherichia coli K-12 as additional tools in chemical production, Appl.Microbiol. Biotechnol DOI: 10.1007/s00253-012-4474-5 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]
• Jan-Karl Guterl, Daniel Garbe, Jörg Carsten, Fabian Steffler, Bettina Sommer, Steven Reiße, Anja Philipp, Martina Haack, Broder Rühmann, Ulrich Kettling, Thomas Brück & Volker Sieber (2012) Cell-free metabolic engineering – Production of chemicals via minimized reaction cascades ChemSusChem, DOI: 10.1002/cssc.20120036

Patents:

• Volker Sieber, André Pick, Broder Rühmann (2011) Synthetic enzymatic pathway for the production of chemicals with reduced number of functional groups EP11159592.2
• Volker Sieber, Katrin Grammann, Broder Rühmann, Thomas Haas (2010) Method for producing multicyclical ring systems carrying amino groups. WO 2010089171
• Volker Sieber, Johannes Kaiser, Broder Rühmann et al. (2007). Manufacture of isoprenoids with microorganisms expressing plant genes for enzymes of isoprenoid biosynthesis, Evonik Degussa GmbH, Germany WO 2009030654
• Volker Sieber, Astrid Jäger, Broder Rühmann (2007) Fermentation of biopolymers with lower acyl group content using microorganisms with increased acylase activity, Evonik Degussa GmbH, Germany DE 102007041861