Book chapters and patents can be found below./a>.

Journal article


  • Teshima, Mariko; Willers, Vivian Pascal; Sieber, Volker (2023)  Cell-free enzyme cascades – application and transition from development to industrial implementation Current Opinion in Biotechnology 79  102868 10.1016/j.copbio.2022.102868 9100492
  • Pangotra, Dhananjai; Roth, Arne; Sieber, Volker; Vieira, Luciana (2023)  Electrochemical Water Oxidation to Hydrogen Peroxide on Bipolar Plates ACS Sustainable Chemistry & Engineering 11 7 2680-2685 10.1021/acssuschemeng.2c06314 
  • Willers, Vivian Pascal; Beer, Barbara; Sieber, Volker  (2023)  Integrating carbohydrate and C1 utilization for chems. production ChemSusChem  e202202122 10.1002/cssc.202202122
  • Al-Shameri, Ammar; Schmermund, Luca; Sieber, Volker (2023)  Engineering approaches for O2-dependent enzymes Current Opinion in Green and Sustainable Chemistry 40  100733 10.1016/j.cogsc.2022.100733 


  • Bayaraa, Tenuun; Lonhienne, Thierry; Sutiono, Samuel; Melse, Okke; Brueck, Thomas B.; Marcellin, Esteban; Bernhardt, Paul V.; Boden, Mikael; Harmer, Jeffrey R.; Sieber, Volker; Guddat, Luke W.; Schenk, Gerhard (2023)  Structural and Functional Insight into the Mechanism of the Fe-S Cluster-Dependent Dehydratase from Paralcaligenes ureilyticus Chemistry – A European Journal 29 9 e202203140 10.1002/chem.202203140 9513783  
  • Sinzinger, K., Schieder, D., Rühmann, B., & Sieber, V. (2022)  Towards a cyanobacterial biorefinery: Carbohydrate fingerprint, biocomposition and enzymatic hydrolysis of Nostoc biomass. Algal Research 65  102744 10.1016/j.algal.2022.102744
  • Pangotra, Dhananjai; Csepei, Lenard-Istvan; Roth, Arne; Sieber, Volker; Vieira, Luciana (2022)  Anodic generation of hydrogen peroxide in continuous flow Green Chemistry 24 20 7931-7940 10.1039/d2gc02575b 
  • Wegat, V., Fabarius, J.T., Sieber, V.  (2022)  Synthetic methylotrophic yeasts for the sustainable fuel and chemical production Biotechnology for Biofuels and Bioproducts 15 1 pp. 113 DOI: 10.1186/s13068-022-02210-1
  • Roeder, H., Kumar, K., Fuechsl, S., Sieber, V. (2022)  Ex-ante life cycle assessment and scale up: A protein production case study Journal of Cleaner Production 376  134329 DOI: 10.1016/j.jclepro.2022.134329
  • Bayaraa, T., Gaete, J., Sutiono, S., Kurz, J., Lonhienne, T., Harmer, J.R., Bernhardt, P.V., Sieber, V., Guddat, L., Schenk, G. (2022)  Dihydroxy-Acid Dehydratases From Pathogenic Bacteria: Emerging Drug Targets to Combat Antibiotic Resistance Chemistry – A European Journal 28 44 pp. e202200927 DOI: 10.1002/chem.202200927
  • Foley, G., Mora, A., Ross, C.M., Bottoms, S., Sutzl, L., Lamprecht, M.L., Zaugg, J., Essebier, A., Balderson, B., Newell, R., Thomson, R.E.S., Kobe, B., Barnard, R.T., Guddat, L., Schenk, G., Carsten, J., Gumulya, Y., Rost, B., Haltrich, D., Sieber, V., Gillam, E.M.J., Boden, M. (2022)  Engineering indel and substitution variants of diverse and ancient enzymes using Graphical Representation of Ancestral Sequence Predictions (GRASP) PLoS Computational Biology 18 10 pp. e1010633 DOI: 10.1371/journal.pcbi.1010633
  • Zachos, I., Guener, S., Essert, A., Lommes, P., Sieber, V.  (2022)  Boosting artificial nicotinamide cofactor systems Chemical Communications 58 85 pp. 11945-11948 DOI: 10.1039/d2cc03423a
  • Paschalidis, L., Beer, B., Sutiono, S., Sieber, V., Burger, J. (2022)  Design of enzymatic cascade reactors through multi-objective dynamic optimization. Biochemical Engineering Journal 181  DOI:10.1016/j.bej.2022.108384
  • Melse, O., Sutiono, S., Haslbeck, M., Schenk, G., Antes, I., Sieber, V. (2022)  Structure‐Guided Modulation of the Catalytic Properties of [2Fe− 2S]‐Dependent Dehydratases. ChemBioChem   DOI: 10.1002/cbic.202200088
  • You Lv, Shan Zheng, Adi Goldenzweig, Fengjiang Liu, Yan Gao, Xiuna Yang, Ajit Kandale, Ross P McGeary, Simon Williams, Bostjan Kobe, Mark A Schembri, Michael J Landsberg, Bin Wu, Thomas B Brück, Volker Sieber, Mikael Boden, Zihe Rao, Sarel J Fleishman, Gerhard Schenk, Luke W Guddat (2022)  Enhancing the Thermal and Kinetic Stability of Ketol-Acid Reductoisomerase, a Central Catalyst of a Cell-Free Enzyme Cascade for the Manufacture of Platform Chemicals Applied Biosciences 1 2 163-178. DOI: 10.3390/applbiosci1020011
  • Zachos, I., Genth, R., Sutiono, S., Marczynski, M., Lieleg, O., Sieber, V. (2022)  Hot Flows: Evolving an Archaeal Glucose Dehydrogenase for Ultrastable Carba-NADP+ Using Microfluidics at Elevated Temperatures ACS Catalysis 12  1841-1846. DOI: 10.1021/acscatal.1c04320
  • Pangotra, D., Csepei, L.-I., Roth, A., Ponce de León, C., Sieber, V., Vieira, L. (2022)  Anodic production of hydrogen peroxide using commercial carbon materials Applied Catalysis B: Environmental 303  no. 120848.  DOI: 10.1016/j.apcatb.2021.120848
  • Schilling, C., Klau, L.J., Aachmann, F.L., Rühmann, B., Schmid, J., Sieber, V. (2022)  Structural elucidation of the fucose containing polysaccharide of Paenibacillus polymyxa DSM 365 Carbohydrate Polymers 278  no. 118951.  DOI: 10.1016/j.carbpol.2021.118951
  • Rath, T., Rühmann, B., Schmid, J., Sieber, V. (2022)  Systematic optimization of exopolysaccharide production by Gluconacetobacter sp. and use of (crude) glycerol as carbon source Carbohydrate Polymers 276 no. 118769.  DOI: 10.1016/j.carbpol.2021.118769


  • Schilling, C., et al., Structural elucidation of the fucose containing polysaccharide of Paenibacillus polymyxa DSM 365. Carbohydrate Polymers, 2022. 278: p. 118951.
  • Rath, T., et al., Systematic optimization of exopolysaccharide production by Gluconacetobacter sp. and use of (crude) glycerol as carbon source. Carbohydrate Polymers, 2022. 276: p. 118769.
  • A. M. Chánique, N. Dimos, I. Drienovská, E. Calderini, M. P. Pantín, C. P. O. Helmer, M. Hofer, V. Sieber, L. P. Parra, B. Loll, R. Kourist (2021),A structural view on the stereospecificity of plant borneol‐type dehydrogenases, ChemCatChem 2021, 13, 2262. doi:10.1002/cctc.202100110
  • Sutiono, Samuel & Pick, André & Sieber, Volker. (2021). Converging conversion – using promiscuous biocatalysts for the cell-free synthesis of chemicals from heterogeneous biomass. Green Chemistry. 23. 10.1039/D0GC04288A.
  • Richter M, Vieira L, Sieber V. Sustainable Chemistry – An Interdisciplinary Matrix Approach. ChemSusChem. 2021 Jan 7;14(1):251-265. doi: 10.1002/cssc.202001327. Epub 2020 Nov 20. PMID: 32945148.
  • Zachos, I., Döring, M., Tafertshofer, G., Simon, R.C. and Sieber, V. (2021), carba Nicotinamide Adenine Dinucleotide Phosphate: Robust Cofactor for Redox Biocatalysis. Angew. Chem. Int. Ed..
  • Hofer, M., Diener, J., Begander, B. et al. Engineering of a borneol dehydrogenase from P. putida for the enzymatic resolution of camphor. Appl Microbiol Biotechnol 105, 3159–3167 (2021).
  • Iwanow, M.; Seidler, J.; Vieira, L.; Kaiser, M.; Van Opdenbosch, D.; Zollfrank, C.; Gärtner, T.; Richter, M.; König, B.; Sieber, V. Enhanced C2 and C3 Product Selectivity in Electrochemical CO2 Reduction on Carbon-Doped Copper Oxide Catalysts Prepared by Deep Eutectic Solvent Calcination. Catalysts 2021, 11, 542.
  • Calderini, E., Drienovska, I., Myrtollari, K., Pressnig, M., Sieber, V., Schwab, H., Kourist, R. and Hofer, M. (2021), Simple plug-in synthetic step for the synthesis of (-)-camphor from renewable starting materials. ChemBioChem. Accepted Author Manuscript.
  • Fabarius, J.F.; Wegat, V., Roth, A. and Sieber, V., (2021), Synthetic Methylotrophy in Yeasts: Towards a Circular Bioeconomy. Trends in Biotechnology, 39 (4), 348-358.
  • Medina-Cabrera, E.V.; Gansbiller, M.; Rühmann, B.; Schmid, J ., Sieber V. (2021) Rheological characterization of Porphyridium sordidum and Porphyridium purpureum exopolysaccharides. Carbohydrate Polymers 253, 117237.
  • Begander B., Huber A., Sperl J., Sieber V., (2021) Development of a Cofactor Balanced, Multi Enzymatic Cascade Reaction for the Simultaneous Production of L-Alanine and L-Serine from 2-Keto-3-deoxy-gluconate Catalysts 11 (1), 31.


  • Medina-Cabrera, E.V.; Rühmann, B.; Schmid, J ., Sieber V. (2020) Characterization and comparison of Porphyridium sordidum and Porphyridium purpureum concerning growth characteristics and polysaccharide production. Algal Research 49, 101931.
  • Medina-Cabrera, E.V.; Rühmann, B.; Schmid, J ., Sieber V. (2020) Optimization of growth and EPS production in two Porphyridum strains. Bioresource Technology Reports 11, 100486.
  • Schilling, C.; Koffas, M.A.G.; Sieber, V., Schmid J. (2020) Novel Prokaryotic CRISPR-Cas12a-Based Tool for Programmable Transcriptional Activation and Repression ACS Synthetic Biology 9 (12), 3353-3363.
  • Sutiono, S., Teshima, M., Beer, B., Schenk, G., Sieber, V. (2020) Enabling the direct enzymatic dehydration of D-glycerate to pyruvate as the key step in synthetic enzyme cascades used in the cell-free production of fine chemicals. ACS Catalysis 10 (5), 3110–3118. https://
  • Sutiono, S.; Siebers, B.; Sieber, V., (2020), Characterization of highly active 2‐keto‐3‐deoxy‐Larabinonate and 2‐keto‐3‐deoxy‐D‐xylonate dehydratases in terms of the biotransformation of hemicellulose sugars to chemicals. Appl. Microbiol. Biotechnol. 104 (16), 7023‐7035. 
  • Schilling, C.; Ciccone, R.; Sieber, V.; Schmid, J., (2020), Engineering of the 2,3‐butanediol pathway of Paenibacillus polymyxa DSM 365. Metab. Eng. 61, 381‐388.
  • Schilling, C.; Badri, A.; Sieber, V.; Koffas, M.; Schmid, J., (2020), Metabolic engineering for production of functional polysaccharides. Curr. Opin. Biotechnol. 66, 44‐51.
  • Iwanow, M.; Gaertner, T.; Sieber, V.; Koenig, B., (2020), Activated carbon as catalyst support: precursors, preparation, modification and characterization. Beilstein J. Org. Chem. 16, 1188‐1202.
  • Gmelch, T. J.; Sperl, J. M.; Sieber, V., (2020), Molecular dynamics analysis of a rationally designed aldehyde dehydrogenase gives insights into improved activity for the non‐native cofactor NAD+. ACS Synth. Biol. 9 (4), 920‐929.
  • Gansbiller, M.; Schmid, J.; Sieber, V., (2020), Rheology of sphingans in EPS‐surfactant systems. Carbohydr. Polym. 248, 116778.
  • Bauer, D.; Zachos, I.; Sieber, V., (2020), Production of Propene from n‐Butanol: A Three‐Step Cascade Utilizing the Cytochrome P450 Fatty Acid Decarboxylase OleTJE. ChemBioChem 21(22)  3273-3281.
  • Richter, M.; Vieira, L.; Sieber, V., (2020), Sustainable Chemistry ‐ An Interdisciplinary Matrix Approach. ChemSusChem. 14(1) 251-265.
  • Stockmann, P.N., Van Opdenbosch, D., Poethig, A. et al. Biobased chiral semi-crystalline or amorphous high-performance polyamides and their scalable stereoselective synthesis. Nat Commun 11, 509 (2020).
  • Drienovska, I., Kolanovic, D., Chanique. A., Sieber, V., Hofer, M., Kourist R., Molecular cloning and functional characterization of a two highly stereoselective borneol dehydrogenases from Salvia officinalis L. Phytochemistry (2020) Vol. 172, 112227.
  • Bohlen B., Wastl D., Radomski J., Sieber V., & Vieira L., Electrochemical CO2 reduction to formate on indium catalysts prepared by electrodeposition in deep eutectic solvents, Electrochemistry Communications, (2020) 110,106597, doi:



  • Sutiono S., Satzinger K., Pick A., Carsten Jörg., & Sieber V.,To beat the heat – engineering of the most thermostable pyruvate decarboxylase to date, RSC advances, 2019, 9, 29743-29746,
  • Zachos I, Nowak C, Sieber V. Biomimetic cofactors and methods for their recycling. Current Opinion in Chemical Biology, (2019)   49, pp 59-66 doi: 10.1016/j.cbpa.2018.10.003
  • Gansbiller M, Schmid J, Sieber V  In-depth rheological characterization of genetically modified xanthan-variants Journal of Carbohydrate Polymers (2019) Vol. 213.  doi: 10.1016/j.carbpol.2019.02.055
  • Stockmann, PN, Pastoetter DL, Woelbing M, Falcke C, Winnacker M, Strittmatter H, Sieber V New Bio‐Polyamides from Terpenes: α‐Pinene and (+)‐3‐Carene as Valuable Resources for Lactam Production.Macromolecular rapid communications (2019) 1800903.   doi: 10.1002/marc.201800903
  • Gmelch T J; Sperl J M; Sieber V . Optimization of a reduced enzymatic reaction cascade for the production of L-alanine. Scientific reports (2019) 9(1), 11754, doi: 10.1038/s41598-019-48151-y
  • Perry, S C; Pangotra, D; Vieira, L; Csepei, LI; Sieber, V; Wang, L; Ponce de Leon, C; Walsh, FC.Electrochemical synthesis of hydrogen peroxide from water and oxygen. Nature Reviews Chemistry (2019) 3(7), 442-458. DOI: 10.1038/s41570-019-0110-6
  • Schaeper, S; Wendt, H; Bamberger, J; Sieber, V; Schmid, J; Becker, A. A bifunctional UDP-sugar 4-epimerase supports biosynthesis of multiple cell surface polysaccharides in Sinorhizobium meliloti. Journal of Bacteriology (2019), 201(10), e00801. DOI: 10.1128/JB.00801-18
  • Haslboeck, M; Klotz, M; Sperl J M; Sieber, V; Zollfrank, C; Van Opdenbosch, D. Mechanical and Thermal Properties of Mixed-Tacticity Polyhydroxybutyrates and Their Association with Iso and Atactic Chain Segment Length Distributions. Macromolecules (2019), 52(14), pp. 5407 – 5418. . DOI:10.1021/acs.macromol.9b00931
  • Shelat, K. J.; Adiamo, O. Q.; Mantilla, S. M. O.; Smyth, H. E.; Tinggi, U.; Hickey, S.; Rühmann, B.; Sieber, V.; Sultanbawa, Y., (2019), Overall nutritional and sensory profile of different species of Australian wattle seeds (Acacia spp.): potential food sources in the arid and semi‐arid regions. Foods 8 (10), 482.


  • Dorrstein J; Zollfrank C; Scholz R; Walther F; Schwarz D; Schieder D; Sieber V. Dataset on the structural characterization of organosolv lignin obtained from ensiled Poaceae grass and load dependent molecular weight changes during thermoplastic processing. Data in brief (2018), pp. 17647 – 17652.  DOI: 10.1016/j.dib.2018.01.060
  • Haslboeck, M; Klotz, M; Steiner, L; Sperl, J M; Sieber, V; Zollfrank, C; Van Opdenbosch,D. Stuctures of Mixed-Tacticity Polyhdroxybutyrates. Macromolecules (2018), 51(14), pp. 5001 – 5010.  DOI: 10.1021/acs.macromol.8b01047 
  • Ranganathan S, Sieber V  Recent Advances in the Direct Synthesis of Hydrogen Peroxide Using Chemical Catalysis— A Review, Catalysts (2018) 8(9), 379 doi: 10.3390/catal8090379
  • Schwarz D, Schoenenwald AKJ, Dörrstein J, Sterba J, Kahoun D, Fojtíková P, Vilímek J, Schieder D, Zollfrank C, Sieber V Biosynthesis of poly-3-hydroxybutyrate from grass silage by a two-stage fermentation process based on an integrated biorefinery concept.   Bioresource Technology, (2018), Volume 269, pp 237-245, doi: 10.1016/j.biortech.2018.08.064
  • Sutiono S, Carsten J, Sieber V. Structure‐Guided Engineering of α‐Keto Acid Decarboxylase for the Production of Higher Alcohols at Elevated Temperature  ChemSusChem, (2018), 11, 3335.
    doi: 10.1002/cssc.201800944
  • Beer B, Pick A, Döring M, Lommes P, Sieber V. Substrate scope of a dehydrogenase from Sphingomonas species A1 and its potential application in the synthesis of rare sugars and sugar derivatives  Microbial Biotechnology (2018) 11(4), 747–758 doi:10.1111/1751-7915.13272
  • Sperl JM and Sieber V  Multienzyme Cascade Reactions—Status and Recent Advances. ACS Catal., (2018) 8, pp 2385–2396
  • Ortiz-Tena JG, Rühmann B and Sieber V Colorimetric Determination of Sulfate via an Enzyme Cascade for High-Throughput Detection of Sulfatase Activity. Anal. Chem., (2018) 90 (4), pp 2526–2533
  • Rütering M, Schmid J, Gansbiller M, Braun A, Kleinen J, Schilling M, and Sieber V. Rheological characterization of the exopolysaccharide Paenan in surfactant systems. Carbohydrate Polymers (2018), 181: p. 719-726.


  • Hohagen H, Schwarz D, Schenk G, Guddat LW, Schieder D, Carsten J, Sieber V Deacidification of grass silage press juice by continuous production of acetoin from its lactate via an immobilized enzymatic reaction cascade Bioresource Technology (2017)  245(A), 1084-1092. doi:10.1016/j.biortech.2017.08.203
  • Rütering M, Cress BF, Schilling M, Rühmann B, Koffas M A G, Sieber V, and Schmid J, Tailor-made exopolysaccharides—CRISPR-Cas9 mediated genome editing in Paenibacillus polymyxa. Synthetic Biology, (2017), 2(1): p. ysx007-ysx007. doi:10.1093/synbio/ysx007
  • Funk I, Sieber V, Schmid J  Effects of glucose concentration on 1,18-cis-octadec-9-enedioic acid biotransformation efficiency and lipid body formation in Candida tropicalis. Scientific Reports (2017) 7(1): 13842. doi:10.1038/s41598-017-14173-7
  • Iwanow M, Finkelmeyer J, Soeldner A, Kaiser M, Gaertner T, Sieber V, Koenig B (2017) Preparation of Supported Palladium Catalysts using Deep Eutectic Solvents Chemistry – A European Journal, 23(51), 12467-12470. doi:10.1002/chem.201702790
  • Nowak C, Pick A, Csepei L I, Sieber V  Characterization of Biomimetic Cofactors According to Stability, Redox Potentials, and Enzymatic Conversion by NADH Oxidase from Lactobacillus pentosus. ChemBioChem (2017doi:10.1002/cbic.201700258
  • Ranganathan S & Sieber, V. Development of semi-continuous chemo-enzymatic terpene epoxidation: combination of anthraquinone autooxidation and the lipase-mediated epoxidation process. Reaction Chemistry & Engineering (2017), 2(6), pp. 885 – 895. DOI: 10.1039/C7RE00112F
  • Inprakhon P, Wongthongdee N, Amornsakchai T, Pongtharankul T, Sunintaboon P, Wiemann L O, Durand A, Sieber V  Lipase-catalyzed synthesis of sucrose monoester: Increased productivity by combining enzyme pretreatment and non-aqueous biphasic medium Journal of Biotechnology, (2017) 259, 182-190. doi:10.1016/j.jbiotec.2017.07.021
  • Roth S, Funk I, Hofer M, Sieber V  Chemo-enzymatic synthesis of a novel borneol-based polyester. ChemSusChem (2017) 10(18)
    doi: 10.1002/cssc.201701146
  • Funk I, Rimmel N, Schorsch C, Sieber V, Schmid J  Production of dodecanedioic acid via biotransformation of low cost plant-oil derivatives using Candida tropicalis, Journal of Industrial Microbiology & Biotechnology, (2017) 1-12 doi: 10.1007/s10295-017-1972-6
  • Ranganathan S, Zeitlhofer S, Sieber V,  Development of a lipase-mediated epoxidation process for monoterpenes in choline chloride-based deep eutectic solvents Green Chemistry, (2017) 19(11), 2576-2586. doi:10.1039/C7GC01127J
  • Roth T, Beer B, Pick A, Sieber V, Thermostabilization of the uronate dehydrogenase from Agrobacterium tumefaciens by semi-rational design AMB Express (2017), 7(1), 1-9. doi:10.1186/s13568-017-0405-2
  • Obst U, Lu TK, Sieber V,  A Modular Toolkit for Generating Pichia pastoris Secretion Libraries ACS Synthetic Biology, (2017) 6(6), 1016-1025. doi:10.1021/acssynbio.6b00337
  • Beer B, Pick A, Sieber V,  In vitro metabolic engineering for the production of a-ketoglutarate Metabolic Engineering, (2017) 40, 5-13. doi:10.1016/j.ymben.2017.02.011
  • Nowak C, Pick A, Lommes P., and Sieber V  Enzymatic Reduction of Nicotinamide Biomimetic Cofactors Using an Engineered Glucose Dehydrogenase – Providing a Regeneration System for Artificial Cofactors ACS Catal. (2017) doi: 10.1021/acscatal.7b00721
  • Koenig S, Rühmann B, Sieber V, Schmid J  Quantitative Assay of β-(1,3)–β-(1,6)–Glucans from Fermentation Broth Using Aniline Blue. Carbohydr Polym (2017). doi:10.1016/j.carbpol.2017.06.047
  • Helfricht N, Doblhofer E, Bieber V, Lommes P, Sieber V, Scheibel T, Papastavrou G  Probing the adhesion properties of alginate hydrogels: a new approach towards the preparation of soft colloidal probes for direct force measurements. Soft Matter. (2017)


  • Reisse, S; Bruck, T; Haack, M; Garbe, D; Sommer, B; Steffler, F; Carsten, J; Sieber, V; Bohnen F. In Vitro Bioconversion of Pyruvate to n-Butanol with Minimized Cofactor Utilization. Frontiers in bioengineering and biotechnology (2016), Volume 4 Article 74. DOI: 10.3389/fbioe.2016.00074
  • Lv, Y; Kandale, A; Wun, S J; McGeary, R P.; Williams, S J.; Kobe, B; Sieber, V; Schembri, M A.; Schenk, G; Guddat, L W. Crystal structure of Mycobacterium tuberculosis ketol-acid reductoisomerase at 1.0 Å resolution – a potentialtarget for anti-tuberculosis drug discovery. FEBS Journal (2016), 283(7), pp. 1184-1196. DOI: 10.1111/febs.13672
  • Tadrowski, S; Pedroso, M M.; Sieber, V; Larrabee, J A.; Guddat, L W.; Schenk, G. Metal ions play an essential catalytic role in the mechanism of ketol-acid reductoisomerase. Chemistry – A European Journal (2016), 22(22), pp. 7427-7436 DOI: 10.1002/chem.201600620
  • Schulze, C; Reinhardt, J; Wurster, M; Ortiz-Tena, J G; Sieber, V; Mundt, S. A one-stage cultivation process for lipid- and carbohydrate-rich biomass of Scenedesmus obtusiusculus based on artificial and natural water sources. Bioresource Technology (2016), 218, pp. 498-504 DOI: 10.1016/j.biortech.2016.06.109
  • Loscar ME, Huptas C, Wenning M, Sieber V, Schmid JDraft Genome Sequence of Lysinibacillus xylanilyticus SR-86. Genome Announcements, (2016) 4(6):e01317-01316. doi:10.1128/genomeA.01317-16
  • Pick A, Beer B, Hemmi R, Momma R, Schmid J, Miyamoto K, Sieber V  Identification and characterization of two new 5-keto-4-deoxy-D-Glucarate Dehydratases/Decarboxylases. BMC Biotechnology, (2016) 16(1): p. 80.doi: 10.1186/s12896-016-0308-3
  • Sperl JM, Carsten JM, Guterl J-K, Lommes P, Sieber V  Reaction Design for the Compartmented Combination of Heterogeneous and Enzyme Catalysis. ACS Catalysis, (2016) 6(10):6329–6334. doi:10.1021/acscatal.6b01276
  • Ranganathan S, Tebbe J, Wiemann LO, Sieber V  Optimization of the lipase mediated epoxidation of monoterpenes using the design of experiments—Taguchi method. Process Biochemistry (2016) doi:10.1016/j.procbio.2016.07.005
  • Schwarz D, Dörrstein J, Kugler S, Schieder D, Zollfrank C, Sieber V  Integrated biorefinery concept for grass silage using a combination of adapted pulping methods for advanced saccharification and extraction of lignin. Bioresource Technology,(2016) 216:462-470.doi:10.1016/j.biortech.2016.05.092
  • Ortiz-Tena JG, Rühmann B, Schieder D, Sieber V  Revealing the diversity of algal monosaccharides: Fast carbohydrate fingerprinting of microalgae using crude biomass and showcasing sugar distribution in Chlorella vulgaris by biomass fractionation. Algal Research, (2016) 17:227-235.doi:10.1016/j.algal.2016.05.008
  • Rütering M, Schmid J, Rühmann B, Schilling M, Sieber V  Controlled production of polysaccharides – exploiting nutrient supply for levan and heteropolysaccharide formation in Paenibacillus sp.. Carbohydrate Polymers (2016) 148, 326-334.
  • Rühmann B, Schmid J, Sieber V  Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis. J Vis Exp, (2016) 110(e53249).doi:10.3791/53249
  • Schrepfer P, Buettner A, Goerner C, Hertel M, van Rijn J, Wallrapp F, Eisenreich W, Sieber V, Kourist R, Brück T  Identification of amino acid networks governing catalysis in the closed complex of class I terpene synthases. Proceedings of the National Academy of Sciences, (2016) 113(8), E958-E967.
  • Schmid J, Farina J, Rehm B, Sieber V  Editorial: “Microbial Exopolysaccharides: From genes to applications”. Frontiers in Microbiology, (2016) 7.doi 10.3389/fmicb.2016.00308
  • Schmid, J., Heider, D., Wendel, N.J., Sperl, N., Sieber, V. . Bacterial Glycosyltransferases: Challenges and opportunities of a highly diverse enzyme class toward tailoring natural products. Frontiers in Microbiology, (2016) 7.doi: 10.3389/fmicb.2016.00182


  • Lermak, L; Degtjarik, O; Steffler, F; Sieber, V; Kuta Smatanova, I. Crystallization behaviour of glyceraldehyde dehydrogenase from Thermoplasma acidophilum. Acta Crystallographica, Section F: Structural Biology Communications (2015), 71(12), pp. 1475-1480. DOI: 10.1107/s2053230x15020270
  • Carsten, J. M., Schmidt, A. & Sieber, V. . Characterization of recombinantly expressed dihydroxy-acid dehydratase from Sulfobus solfataricus-A key enzyme for the conversion of carbohydrates into chemicals. J Biotechnol (2015) 211, 31-41.
    doi: 10.1016/j.jbiotec.2015.06.384
  • Nowak, C., Beer, B., Pick, A., Roth, T., Lommes, P. & Sieber, V. (2015). A water-forming NADH oxidase from Lactobacillus pentosus suitable for the regeneration of synthetic biomimetic cofactors. Frontiers in Microbiology 6.
    doi: 10.3389/fmicb.2015.00957
  • Rühmann, B., Schmid, J. & Sieber, V. (2015). Methods to identify the unexplored diversity of microbial exopolysaccharides. Frontiers in Microbiology 6.
    doi: 10.3389/fmicb.2015.00565
  • Schmid, J., Sieber, V. & Rehm, B. (2015). Bacterial exopolysaccharides: Biosynthesis pathways and engineering strategies. Frontiers in Microbiology 6.
    doi: 10.3389/fmicb.2015.00496
  • Pick, A., Schmid, J. & Sieber, V. (2015). Characterization of uronate dehydrogenases catalysing the initial step in an oxidative pathway. Microbial Biotechnology 8, 633-643.
    doi: 10.1111/1751-7915.12265
  • de Reese, J., Sperl, N., Schmid, J., Sieber, V. & Plank, J. (2015). Effect of biotechnologically modified alginates on LDH structures. Bioinspired, Biomimetic and Nanobiomaterials 3, 1-13.
    doi: 10.1680/bbn.14.00032
  • Schmid, J. & Sieber, V. (2015). Enzymatic Transformations Involved in the Biosynthesis of Microbial Exo-polysaccharides Based on the Assembly of Repeat Units. ChemBioChem 16, 1141-1147.
    doi: 10.1002/cbic.201500035
  • Ranganathan, S., Gärtner, T., Wiemann, L. O. & Sieber, V. (2015). A one pot reaction cascade of in situ hydrogen peroxide production and lipase mediated in situ production of peracids for the epoxidation of monoterpenes. Journal of Molecular Catalysis B: Enzymatic 114, 72-76.
    doi: 10.1016/j.molcatb.2014.12.008
  • Rühmann, B., Schmid, J. & Sieber, V. (2015). High throughput exopolysaccharide screening platform: From strain cultivation to monosaccharide composition and carbohydrate fingerprinting in one day. Carbohydrate Polymers 122, 212-220.
    doi: 10.1016/j.carbpol.2014.12.021
  • Zachos, I., Gaßmeyer, S. K., Bauer, D., Sieber, V., Hollmann, F. & Kourist, R. (2015). Photobiocatalytic decarboxylation for olefin synthesis. Chemical Communications 51, 1918-1921.
    doi: 10.1039/C4CC07276F


  • Schmid, J., Sperl, N. & Sieber, V. (2014). A comparison of genes involved in sphingan biosynthesis brought up to date. Applied Microbiology and Biotechnology 98, 7719-7733.
    doi: 10.1007/s00253-014-5940-z
  • Tosstorff, A., Dennig, A., Ruff, A. J., Schwaneberg, U., Sieber, V., Mangold, K.-M., Schrader, J. & Holtmann, D. (2014). Mediated electron transfer with monooxygenases—Insight in interactions between reduced mediators and the co-substrate oxygen. Journal of Molecular Catalysis B: Enzymatic 108, 51-58.
    doi: 10.1016/j.molcatb.2014.06.011
  • Pick, A., Ott, W., Howe, T., Schmid, J. & Sieber, V. (2014). Improving the NADH-cofactor specificity of the highly active AdhZ3 and AdhZ2 from Escherichia coli K-12. Journal of Biotechnology 189, 157-165.
    doi: 10.1016/j.jbiotec.2014.06.015
  • Schmid, J., Koenig, S., Pick, A., Steffler, F., Yoshida, S., Miyamoto, K. & Sieber, V. (2014). Draft Genome Sequence of Kozakia baliensis SR-745, the First Sequenced Kozakia Strain from the Family Acetobacteraceae. Genome Announcements 2, e00594-14
    doi: 10.1128/genomeA.00594-14
  • Reiter, J., Pick, A., Wiemann, L., Schieder, D. & Sieber, V. (2014). Novel Natural NADH and NADPH Dependent Glutathione Reductase as Tool in Biotechnological Applications. JSM Biotechnol Bioeng 2, 1028.
    doi: link zum Artikel
  • Rühmann, B., Schmid, J. & Sieber, V. (2014). Fast carbohydrate analysis via liquid chromatography coupled with ultra violet and electrospray ionization ion trap detection in 96-well format. Journal of Chromatography A 1350, 44-50.
    doi: 10.1016/j.chroma.2014.05.014
  • Schmid, J., Koenig, S., Rühmann, B., Rütering, M. & Sieber, V. (2014). Biosynthese und Genomik mikrobieller Polysaccharide. BIOspektrum 20, 288-290.
    doi: 10.1007/s12268-014-0443-0
  • Pöhnlein, M., Slomka, C., Kukharenko, O., Gärtner, T., Wiemann, L. O., Sieber, V., Syldatk, C. & Hausmann, R. (2014). Enzymatic synthesis of amino sugar fatty acid esters. European Journal of Lipid Science and Technology 116, 423-428.
    doi: 10.1002/ejlt.201300380
  • Kourist, R., Guterl, J.-K., Miyamoto, K. & Sieber, V. (2014). Enzymatic Decarboxylation—An Emerging Reaction for Chemicals Production from Renewable Resources. ChemCatChem 6, 689-701.
    doi: 10.1002/cctc.201300881
  • Häßler, T., Schieder, D., Pfaller, R., Faulstich, M. & Sieber, V. (2014). Effects of Selected Yeast Extract Compounds on 2,3-Butanediol Production by Paenibacillus polymyxa DSM 365. Current Biotechnology 3, 157-165.
    doi: 10.2174/2211550102666140227000520
  • Inprakhon, P., Panlawan, P., Pongtharankul, T., Marie, E., Wiemann, L. O., Durand, A. & Sieber, V. (2014). Toward one-pot lipase-catalyzed synthesis of poly(ɛ-caprolactone) particles in aqueous dispersion. Colloids and Surfaces B: Biointerfaces 113, 254-260.
    doi: 10.1016/j.colsurfb.2013.09.011


  • Hofer, M., Strittmatter, H. & Sieber, V. (2013). Biocatalytic Synthesis of a Diketobornane as a Building Block for Bifunctional Camphor Derivatives. ChemCatChem 5, 3351-3357.
    doi: 10.1002/cctc.201300344
  • Steffler, F. & Sieber, V. (2013). Refolding of a Thermostable Glyceraldehyde Dehydrogenase for Application in Synthetic Cascade Biomanufacturing. PLoS ONE 8, e70592.
    doi: 10.1371/journal.pone.0070592
  • Steffler, F., Guterl, J.-K. & Sieber, V. (2013). Improvement of thermostable aldehyde dehydrogenase by directed evolution for application in Synthetic Cascade Biomanufacturing. Enzyme and Microbial Technology 53, 307-314.
    doi: 10.1016/j.enzmictec.2013.07.002
  • Pick, A., Rühmann, B., Schmid, J. & Sieber, V. (2013). Novel CAD-like enzymes from Escherichia coli K-12 as additional tools in chemical production. Applied Microbiology and Biotechnology 97, 5815-5824.
    doi: 10.1007/s00253-012-4474-5
  • Reiter, J., Strittmatter, H., Wiemann, L. O., Schieder, D. & Sieber, V. (2013). Enzymatic cleavage of lignin beta-O-4 aryl ether bonds via net internal hydrogen transfer. Green Chemistry 15, 1373-1381.
    doi: 10.1039/C3GC40295A
  • Flemke, J., Maywald, M. & Sieber, V. (2013). Encapsulation of Living E. coli Cells in Hollow Polymer Microspheres of Highly Defined Size. Biomacromolecules 14, 207-214.
    doi: 10.1021/bm3016362
  • Guterl, J.-K. & Sieber, V. (2013). Biosynthesis “debugged”: Novel bioproduction strategies. Engineering in Life Sciences 13, 4-18.
    doi: 10.1002/elsc.201100231


  • Häßler, T., Schieder, D., Pfaller, R., Faulstich, M. & Sieber, V. (2012). Enhanced fed-batch fermentation of 2,3-butanediol by Paenibacillus polymyxa DSM 365. Bioresource Technology 124, 237-244.
    doi: 10.1016/j.biortech.2012.08.047
  • Wiemann, L., Faltl, C. & Sieber, V. (2012). Lipase-mediated Epoxidation of the Cyclic Monoterpene Limonene to Limonene Oxide and Limonene Dioxide. Zeitschrift für Naturforschung B 67, 1056–1060.
    doi: 10.5560/znb.2012-0167
  • Janker-Obermeier, I., Sieber, V., Faulstich, M. & Schieder, D. (2012). Solubilization of hemicellulose and lignin from wheat straw through microwave-assisted alkali treatment. Industrial Crops and Products 39, 198-203.
    doi: 10.1016/j.indcrop.2012.02.022
  • Guterl, J.-K., Garbe, D., Carsten, J., Steffler, F., Sommer, B., Reiße, S., Philipp, A., Haack, M., Rühmann, B., Koltermann, A., Kettling, U., Brück, T. & Sieber, V. (2012). Cell-Free Metabolic Engineering: Production of Chemicals by Minimized Reaction Cascades. ChemSusChem 5, 2165-2172.
    doi: 10.1002/cssc.201200365
  • Kolb, M., Sieber, V., Amann, M., Faulstich, M. & Schieder, D. (2012). Removal of monomer delignification products by laccase from Trametes versicolor. Bioresource Technology 104, 298-304.
    doi: 10.1016/j.biortech.2011.11.080


  • Schmid, J., Meyer, V. & Sieber, V. (2011). Scleroglucan: biosynthesis, production and application of a versatile hydrocolloid. Applied Microbiology and Biotechnology 91, 937-947.
    doi: 10.1007/s00253-011-3438-5
  • Schmid, J., Muller-Hagen, D., Bekel, T., Funk, L., Stahl, U., Sieber, V. & Meyer, V. (2010). Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii. BMC Genomics 11, 329.
    doi: 10.1186/1471-2164-11-329


  • Vorhölter, F.-J., Schneiker, S., Goesmann, A., Krause, L., Bekel, T., Kaiser, O., Linke, B., Patschkowski, T., Rückert, C., Schmid, J., Sidhu, V. K., Sieber, V., Tauch, A., Watt, S. A., Weisshaar, B., Becker, A., Niehaus, K. & Pühler, A. (2008). The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis. Journal of Biotechnology 134, 33-45.
    doi: 10.1016/j.jbiotec.2007.12.013


  • Butler, T., Alcalde, M., Sieber, V., Meinhold, P., Schlachtbauer, C. & Arnold, F. H. (2003). Functional Expression of a Fungal Laccase in Saccharomyces cerevisiae by Directed Evolution Applied and Environmental Microbiology 69, 987-995.
    doi: 10.1128/AEM.69.2.987-995.2003


  • Martin, A., Sieber, V. & Schmid, F. X. (2001). In-vitro Selection of Highly Stabilized Protein Variants with Optimized Surface. Journal of Molecular Biology 309, 717-726.
    doi: 10.1006/jmbi.2001.4698
  • Sieber, V., Martinez, C. A. & Arnold, F. H. (2001). Libraries of hybrid proteins from distantly related sequences. Nat Biotech 19, 456-460.
    doi: 10.1038/88129


  • Sieber, V., Pluckthun, A. & Schmid, F. X. (1998). Selecting proteins with improved stability by a phage-based method. Nat Biotech 16, 955-960.
    doi: 10.1038/nbt1098-955
  • Heffron, S., Moe, G. R., Sieber, V., Mengaud, J., Cossart, P., Vitali, J. & Jurnak, F. (1998). Sequence Profile of the Parallel β Helix in the Pectate Lyase Superfamily. Journal of Structural Biology 122, 223-235.
    doi: 10.1006/jsbi.1998.3978
  • Schindler, T., Perl, D., Graumann, P., Sieber, V., Marahiel, M. A. & Schmid, F. X. (1998). Surface-exposed phenylalanines in the RNP1/RNP2 motif stabilize the cold-shock protein CspB from Bacillus subtilis. Proteins: Structure, Function, and Bioinformatics 30, 401-406.
    doi: 10.1002/(SICI)1097-0134(19980301)30:4<401::AID-PROT7>3.0.CO;2-L


  • Sieber, V. & Moe, G. R. (1996). Interactions Contributing to the Formation of a β-Hairpin-like Structure in a Small Peptide. Biochemistry 35, 181-188.
    doi: 10.1021/bi950681o


  • Sieber, V., Jurnak, F. & Moe, G. R. (1995). Circular dichroism of the parallel β helical proteins pectate lyase C and E. Proteins: Structure, Function, and Bioinformatics 23, 32-37.
    doi: 10.1002/prot.340230105

Book chapters

  • Schmid, J., B. Rühmann, V. Sieber, L. Romero-Jiménez, J. Sanjuán, and D. Pérez-Mendoza, Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria, in Host-Pathogen Interactions: Methods and Protocols, C. Medina and F.J. López-Baena, Editors. (2018), Springer New York: New York, NY. p. 263-275.
  • Schmid, J., Mueller-Hagen, D., Sieber, V. & Meyer, V. (2013). Nucleic and Protein Extraction Methods for Fungal Exopolysaccharide Producers. In Laboratory Protocols in Fungal Biology (Gupta, V. K., Tuohy, M. G., Ayyachamy, M., Turner, K. M. & O’Donovan, A., eds.), pp. 427-434. Springer New York.
  • Schmid, J., Stahl, U. & Meyer, V. (2009). Genetic and Metabolic Engineering in Filamentous Fungi. In Physiology and Genetics, pp. 377-392.
  • Zuccarello, G., Critchley, A., Smith, J., Sieber, V., Lhonneur, G. & West, J. (2007). Systematics and genetic variation in commercial Kappaphycus and Eucheuma (Solieriaceae, Rhodophyta). In Eighteenth International Seaweed Symposium (Anderson, R., Brodie, J., Onsøyen, E. & Critchley, A., eds.), Vol. 1, pp. 417-425. Springer Netherlands.
  • Sieber, V., Wittmann, E. & Buchholz, S. (2005). Polysaccharide. In Lehrbuch für Angewandte Mikrobiologie (Antranikian, P. G., ed.), pp. 399-410. Springer Verlag.
  • Udit, A. K., Silberg, J. J. & Sieber, V. (2003). Sequence Homology-Independent Protein Recombination (SHIPREC). In Directed Evolution Library Creation (Arnold, F. & Georgiou, G., eds.), Vol. 231, pp. 153-163. Humana Press.
  • Sieber, V. (2003). Selection for Soluble Proteins via Fusion with Chloramphenicol Acetyltransferase. In Directed Enzyme Evolution (Arnold, F. & Georgiou, G., eds.), Vol. 230, pp. 45-55. Humana Press.
  • Martin, A., Schmid, F. X. & Sieber, V. (2003). Proside: A Phage-Based Method for Selecting Thermostable Proteins, Vol. 230, pp. 57-70.
  • Bulter, T., Sieber, V. & Alcalde, M. (2003). Screening Mutant Libraries in Saccharomyces cerevisiae. In Directed Enzyme Evolution (Arnold, F. & Georgiou, G., eds.), Vol. 230, pp. 99-107. Humana Press.


  • Csepei, Lenard-Istvan; Steffler, Fabian; Gaertner, Tobias; Sieber, Volker (2017) Regenerative synthesis of chemical stored energy sources and fine chemicals. WO 2017153396 A1 20170914.
  • Gaertner, Tobias; Kaiser, Manuela; Sieber, Volker; Soeldner, Anika; Koenig, Burkhard; Iwanow, Melanie (2016) Pyrolytic method for the production of particles containing at least one metal species. WO 2016180973 A1 20161117.
  • Sieber, Volker; Pick, Andre; Ruehmann, Broder (2015) Process for the enzymatic production of c4 compounds from c6 substrates, US 9169500 B2 20151027
  • Falcke, Claudia; Strittmatter, Harald; Woelbing, Marion; Sieber, Volker (2016) Terpene lactam-based polyamides. Ger. Offen., DE 102014221061 A1 20160421
  • Kraus, Michael; Koltermann, Andre; Kettling, Ulrich; Garbe, Daniel; Brueck, Thomas; Guterl, Jan-Karl; Sieber, Volker (2014) Cell-free and minimized metabolic reaction cascades for the production of chemicals. Pat. Appl., EP 2700714 A1 20140226.
  • Karau, Andreas; Sieber, Volker; Haas, Thomas; Haeger, Harald; Grammann, Katrin; Buehler, Bruno; Blank, Lars; Schmid, Andreas (2009) Production of ω-aminocarboxylic acids or their lactams by recombinant bacteria PCT Int. Appl., WO 2009077461 A1 20090625
  • Sieber, V., Pick, A. & Rühmann, B. (2011) Synthetic pathway for the production of alcohols or amines. EP2503003 A1
  • Sieber, V., Grammann, K., Rühmann, B., Haas, T., Pfeffer, J., Doderer, K., Rollmann, C., Skerra, A., Rausch, C. & Lerchner, A. (2010) Method for producing multicyclical ring systems carrying amino groups. WO2010089171 A2
  • Koltermann, A., Kettling, U. & Sieber, V. (2010) Process for cell-free production of chemicals. WO2010076305 A1
  • Sieber, V., Kaiser, J., Ruehmann, B., Potgrave, N., Wittmann, E. M., Marx, A., Windhab, N., Ewering, C., Kroll, J., Schulze, G. C., Pruefer, D., Steinbuechel, A., Wurbs, D., Bacher, A., Eisenreich, W., Graewert, T. W., Illarinova, V., Groll, M. & Rohdich, F. (2009) Mikrobiologische herstellung von isoprenoiden. WO2009030654 A1
  • Jäger, A., Rühmann, B. & Sieber, V. (2009) Producing an acylated polymer with a low degree of acylation, especially xanthan gum, comprises culturing a polymer-producing microorganism that has been modified to overexpress an acylase. DE102007041861 A1
  • Jäger, A., Rühmann, B. & Sieber, V. (2007) Herstellung von Polymeren mit reduziertem Acylgehalt. DE102007041861 A1
  • Loetzbeyer, T., Sieber, V., Volland, M. & Wittmann, E. (2005) Verfahren zur enzymatischen herstellung von mono- und diacylglycerid-haltigen emulgatoren. WO2005024036 A3
  • Deck, D. & Sieber, V. (2004) Verfahren zur Detektion und Quantifizierung von Bakterien in einer Probe. DE10311924 A1
  • Sieber, V. (2003) Method for producing gene libraries. WO2002068634 A3
  • Arnold, F. H., Sieber, V. & Zhang, J. H. (2002) Production of functional hybrid genes and proteins. WO2001030998 A9
  • Sieber, V. & Schmid, F. X. (1998) A bacteriophage infectivity assay for rapid screening of proteins to identify variants with increased stability. DE 19980302


Chair of Chemistry of Biogenic Resources

Schulgasse 16
D-94315 Straubing


Prof. Dr. Volker Sieber

Phone: +49 (0) 9421 187-300
Phone: +49 (0) 8161 71 35 91

Team Assistant

Elisabeth Aichner

Phone: +49 (0) 9421 187-301